OxLDL-treated macrophages have an increased <i>Mtb</i> burden.

Primary human macrophages were treated with PBS control, native LDL or oxLDL (1, 10 or 25 μg/ml) overnight and subsequently infected with Mtb H37Rv at a MOI of 10:1. (A) Oil Red O staining of macrophages treated overnight with PBS, 25 μg/ml LDL or 1, 10 and 25 μg/ml oxLDL. Pictures were taken at a 20x magnification. (B) Macrophages were lysed at 24 h post-infection and bacterial load was determined by CFU assay. Results were normalized versus PBS control (n = 8). Individual donors are depicted as dots with group medians. Statistical significance was determined by Wilcoxon signed rank test with post-hoc FDR correction. ** = p < 0.01.