Percentage of macrophages that ingest Mtb.

<p>(A) Macrophages were infected with FITC-labeled wild-type <i>or</i> Δ<i>choD</i> strains for 2 hours. (B and C) Macrophages were treated with IRAK1/4 inhibitor, anti-TLR2 mAb, or anti-CR3 mAb for 1 hour, and then were infected with (B) opsonized or (C) non-opsonized FITC-labeled wild-type or Δ<i>choD</i> strains for 2 hours. In all samples, macrophages with ingested bacteria were counted using a fluorescence microscope. All data are presented as the percentage of macrophages involved in phagocytosis, expressed as means ± SEMs (*p≤0.02, Mtb strain vs. Mtb strain+IRAK1/4 inhibitor or anti-TLR2 mAb or anti-CR3 mAb, # p≤0.03, Δ<i>choD</i>+anti-CR3 mAb vs. Δ<i>choD</i>+anti-TLR2 mAb,; Mann-Whitney <i>U</i> test). Data are presented from five independent experiments. Each experiment was carried out in duplicate.