Intracellular survival of H37Rv in macrophages from healthy volunteers and individuals with T2DM.

<p>Macrophages were infected with H37Rv, a laboratory strain of <i>M</i>.<i>tb</i>, with multiplicity of infection 10:1. One hour post-infection, infected macrophageswere treated with 10 mM and 20 mM NAC and 10 μM and 20 μM <i>l</i>GSH. Infected macrophages were terminated at 1h and 5 days post-infection. Lysates were plated on 7H11 medium with ADC to determine <i>M</i>.<i>tb</i> growth. Data represent means ±SE from 5 healthy individuals (Fig. 9A) and 5 individuals with T2DM (Fig. 9B). Experiments were performed in triplicates. *p<0.05 when comparing H37Rv-infected macrophages 5 day time point results to any other 5 day time point categories while #p<0.05 when comparing 1 hour and 5 day time points of the same treatment category.