Optimization of SVA RT-LAMP reaction conditions.

A): Reaction temperature optimization: M: 2000 bp DNA marker, lanes 1 to 8 represent 57°C, 58°C, 59°C, 60°C, 61°C, 62°C, 63°C, 64°C, for a gradient with 8 temperatures; B): Reaction time optimization: M: 2000 bp DNA marker, lanes 1 to 8 respectively represent reaction times of 30 min, 35 min, 40 min, 45 min, 50 min, 55 min, 60 min, and 65 min, for a gradient with 8 reaction times.