A): RT-LAMP with gel electrophoresis detection: M: 2000 bp DNA marker, lane 1 for positive template (P), lane 2 for negative control (N); B): calcein RT-LAMP detection method: tube 1 for positive template (P), tube 2 for negative control (N); C): RT-LAMP-LFD detection method: strip 1 for positive template (P), strip 2 for negative control (N).</p
<p>(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standar...
<p>(A) Sensitivity assessment performed with a thermocycler. (B) Sensitivity assessment performed wi...
(A)- Visual appearance of amplified product on addition of diluted SYBR Green I for LAMP and DBL-LAM...
M: 2000 bp DNA marker; lanes 1 to 10 are SVA, FMDV, SVDV, VSV, PDCoV, PEDV, TGEV, PRRSV, CSFV and ne...
1: 4.56x10-1 ng/μL; 2: 4.56x10-2 ng/μL; 3: 4.56x10-3 ng/μL; 4: 4.56x10-4 ng/μL; 5: 4.56x10-5 ng/μL; ...
<p>The gel is a 2% agarose E-gel (Invitrogen) with ethidium bromide. The marker (“M”) is a Low Molec...
<p>10 fold dilutions of FMDV containing epithelial suspensions were analysed using the Svanova LFD d...
M, Trans 2K plus II DNA marker; 1, negative control; 2, Positive control. A, D and G, Agarose gel el...
A, D and G, Agarose gel electrophoresis analysis of LAMP, CPA and IMSA reactions; B, E and H, Direct...
<p>Step 1 includes processing of varied specimen (A) through boiling or use of kits to yield a stabl...
This study explores determining the sex of humans from blood stains taken from different surfaces an...
The nucleic acid tests abbreviated as NAT, is a technique requires amplification and detection to pr...
<p>Various amount of template DNA (10, 1, 10<sup>−1</sup>, 10<sup>−2</sup>, 10<sup>−3</sup>, 10<sup>...
<p>Mutant type Panc-1 and QGP-1 samples were serially diluted with wild type Bx and HS766T samples (...
A): Reaction temperature optimization: M: 2000 bp DNA marker, lanes 1 to 8 represent 57°C, 58°C, 59°...
<p>(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standar...
<p>(A) Sensitivity assessment performed with a thermocycler. (B) Sensitivity assessment performed wi...
(A)- Visual appearance of amplified product on addition of diluted SYBR Green I for LAMP and DBL-LAM...
M: 2000 bp DNA marker; lanes 1 to 10 are SVA, FMDV, SVDV, VSV, PDCoV, PEDV, TGEV, PRRSV, CSFV and ne...
1: 4.56x10-1 ng/μL; 2: 4.56x10-2 ng/μL; 3: 4.56x10-3 ng/μL; 4: 4.56x10-4 ng/μL; 5: 4.56x10-5 ng/μL; ...
<p>The gel is a 2% agarose E-gel (Invitrogen) with ethidium bromide. The marker (“M”) is a Low Molec...
<p>10 fold dilutions of FMDV containing epithelial suspensions were analysed using the Svanova LFD d...
M, Trans 2K plus II DNA marker; 1, negative control; 2, Positive control. A, D and G, Agarose gel el...
A, D and G, Agarose gel electrophoresis analysis of LAMP, CPA and IMSA reactions; B, E and H, Direct...
<p>Step 1 includes processing of varied specimen (A) through boiling or use of kits to yield a stabl...
This study explores determining the sex of humans from blood stains taken from different surfaces an...
The nucleic acid tests abbreviated as NAT, is a technique requires amplification and detection to pr...
<p>Various amount of template DNA (10, 1, 10<sup>−1</sup>, 10<sup>−2</sup>, 10<sup>−3</sup>, 10<sup>...
<p>Mutant type Panc-1 and QGP-1 samples were serially diluted with wild type Bx and HS766T samples (...
A): Reaction temperature optimization: M: 2000 bp DNA marker, lanes 1 to 8 represent 57°C, 58°C, 59°...
<p>(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standar...
<p>(A) Sensitivity assessment performed with a thermocycler. (B) Sensitivity assessment performed wi...
(A)- Visual appearance of amplified product on addition of diluted SYBR Green I for LAMP and DBL-LAM...