M: 2000 bp DNA marker; lanes 1 to 10 are SVA, FMDV, SVDV, VSV, PDCoV, PEDV, TGEV, PRRSV, CSFV and negative control (N); A): specificity of RT-LAMP agarose gel electrophoresis assay; B): specificity of calcein RT-LAMP assay; and C): specificity of RT-LAMP-LFD assay.</p
<p>The gel is a 2% agarose E-gel (Invitrogen) with ethidium bromide. The marker (“M”) is a Low Molec...
<p>Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expected ...
<p>Ten-fold serial dilutions starting from 1 ng of genomic DNA (lane 1) down to 1 fg (lane 7) were t...
A): RT-LAMP with gel electrophoresis detection: M: 2000 bp DNA marker, lane 1 for positive template ...
1: 4.56x10-1 ng/μL; 2: 4.56x10-2 ng/μL; 3: 4.56x10-3 ng/μL; 4: 4.56x10-4 ng/μL; 5: 4.56x10-5 ng/μL; ...
<p>10 fold dilutions of FMDV containing epithelial suspensions were analysed using the Svanova LFD d...
<p>(A) Visual examination of LAMP products by adding SYBR Green I. (B) Agarose gel electrophoresis o...
Comparative standard operating protocol of LAMP assay (performed with column extracted DNA) vs. dire...
Sensitivity and specificity of LAMP assay for diagnosis of VL at the two validation sites.</p
(A)- Visual appearance of amplified product on addition of diluted SYBR Green I for LAMP and DBL-LAM...
A, D and G, Agarose gel electrophoresis analysis of LAMP, CPA and IMSA reactions; B, E and H, Direct...
<p>(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standar...
<p>The assay was carried out using different concentrations of RNA from a titrated EV 71 subgenotype...
<p>(A) Sensitivity assessment performed with a thermocycler. (B) Sensitivity assessment performed wi...
A, D and G, Agarose gel electrophoresis analysis of LAMP, CPA and IMSA products; B, E and H, Direct ...
<p>The gel is a 2% agarose E-gel (Invitrogen) with ethidium bromide. The marker (“M”) is a Low Molec...
<p>Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expected ...
<p>Ten-fold serial dilutions starting from 1 ng of genomic DNA (lane 1) down to 1 fg (lane 7) were t...
A): RT-LAMP with gel electrophoresis detection: M: 2000 bp DNA marker, lane 1 for positive template ...
1: 4.56x10-1 ng/μL; 2: 4.56x10-2 ng/μL; 3: 4.56x10-3 ng/μL; 4: 4.56x10-4 ng/μL; 5: 4.56x10-5 ng/μL; ...
<p>10 fold dilutions of FMDV containing epithelial suspensions were analysed using the Svanova LFD d...
<p>(A) Visual examination of LAMP products by adding SYBR Green I. (B) Agarose gel electrophoresis o...
Comparative standard operating protocol of LAMP assay (performed with column extracted DNA) vs. dire...
Sensitivity and specificity of LAMP assay for diagnosis of VL at the two validation sites.</p
(A)- Visual appearance of amplified product on addition of diluted SYBR Green I for LAMP and DBL-LAM...
A, D and G, Agarose gel electrophoresis analysis of LAMP, CPA and IMSA reactions; B, E and H, Direct...
<p>(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standar...
<p>The assay was carried out using different concentrations of RNA from a titrated EV 71 subgenotype...
<p>(A) Sensitivity assessment performed with a thermocycler. (B) Sensitivity assessment performed wi...
A, D and G, Agarose gel electrophoresis analysis of LAMP, CPA and IMSA products; B, E and H, Direct ...
<p>The gel is a 2% agarose E-gel (Invitrogen) with ethidium bromide. The marker (“M”) is a Low Molec...
<p>Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expected ...
<p>Ten-fold serial dilutions starting from 1 ng of genomic DNA (lane 1) down to 1 fg (lane 7) were t...