<p>Real-time PCR optimization varying annealing temperature and primer concentration.</p
<p>Primers used in the study, annealing temperatures used in the PCR and expected sizes of the PCR p...
<p>Real time PCR primer parameters including sequences, product length and melting point temperature...
<p>T<sub>m</sub> means melting temperature; ±SD means standard deviation of the mean value.</p><p>Pr...
<p>Sequences of the primers, and annealing temperatures for the Real Time PCR of the genes studied.<...
<p>Specific primer and probes and optimized temperature conditions for real-time PCR.</p
<p>Primers, annealing temperature and length of PCR products used in differentiation.</p
<p>Primer sequence, annealing temperatures (AT) and PCR product size for the different genes.</p
<p>Nucleotide sequences, product size and annealing temperature of the primers utilized in real-time...
<p>The Quantitative Real Time PCR assay was first performed with varying concentrations of primer an...
<p>Primer sequences and annealing temperatures used for quantitative RT-PCR (qRT-PCR).</p
<p>Primers and annealing temperatures used for the amplification of each target gene.</p
<p>Species-specific and ubiquitous real-time PCR primers for 6 pathogens, the annealing temperature,...
<p>Primer design, annealing temperatures and PCR product lengths of the studied mRNAs.</p
<p>Primers, annealing temperatures and number of cycles used for the amplification of each target ge...
<p>Optimal annealing temperatures (T<sub>A</sub>) were predetermined by gradient PCR, and a T<sub>A<...
<p>Primers used in the study, annealing temperatures used in the PCR and expected sizes of the PCR p...
<p>Real time PCR primer parameters including sequences, product length and melting point temperature...
<p>T<sub>m</sub> means melting temperature; ±SD means standard deviation of the mean value.</p><p>Pr...
<p>Sequences of the primers, and annealing temperatures for the Real Time PCR of the genes studied.<...
<p>Specific primer and probes and optimized temperature conditions for real-time PCR.</p
<p>Primers, annealing temperature and length of PCR products used in differentiation.</p
<p>Primer sequence, annealing temperatures (AT) and PCR product size for the different genes.</p
<p>Nucleotide sequences, product size and annealing temperature of the primers utilized in real-time...
<p>The Quantitative Real Time PCR assay was first performed with varying concentrations of primer an...
<p>Primer sequences and annealing temperatures used for quantitative RT-PCR (qRT-PCR).</p
<p>Primers and annealing temperatures used for the amplification of each target gene.</p
<p>Species-specific and ubiquitous real-time PCR primers for 6 pathogens, the annealing temperature,...
<p>Primer design, annealing temperatures and PCR product lengths of the studied mRNAs.</p
<p>Primers, annealing temperatures and number of cycles used for the amplification of each target ge...
<p>Optimal annealing temperatures (T<sub>A</sub>) were predetermined by gradient PCR, and a T<sub>A<...
<p>Primers used in the study, annealing temperatures used in the PCR and expected sizes of the PCR p...
<p>Real time PCR primer parameters including sequences, product length and melting point temperature...
<p>T<sub>m</sub> means melting temperature; ±SD means standard deviation of the mean value.</p><p>Pr...
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