Staining of collagen fibers.

<p>Collagen fibers were visualized in the aortic sinus by Picro Sirius staining. Significant amounts of collagen fibers were detected in 8 DMSO-treated animals and in 9 JZL184-treated animals (A, B). Quantity and distribution pattern of collagen fibers did not differ between the two groups (C). Data are presented as mean ± standard error of the mean; n = 8–9; p-value as indicated, assessed by student’s t-test. Scale bar, 200 μm. DMSO, dimethyl sulfoxide; JZL184, inhibitor of monoacylglycerol lipase.