Sirius red staining for collagen and IH for macrophages in plaque.

<p>(A) The interstitial collagen content of aorta abdominals plaques was evaluated by Sirius red staining. Collagens are identified by birefringence under polarized light illumination following standard sirius red procedure. (B) IH for macrophage in plaque detected increased macrophages expressed in control group and less in group A2, B2 and C2. A2: triciribine; B2: rapamycin; C2: mTOR-siRNA; D2: control; ^*<i>P</i><0.01 vs control.