Evaluation of collagen fibers from scar tissues by picrosirius staining.

<p>Representative photomicrographs from scar tissues that were subjected to polarization microscopy for visualization of collagen fibers: A. Representative image from the first group with four days of healing. B. From the fourth group with sixteen days of healing, both having received treatment CMC 20 mg/mL+26×10⁶ of ADSCs. C. Shows the collagen fibers in a biopsy of normal skin, not subjected to injury. Optical microscopy. Picrosirius staining (200x). D. The statistical analysis showed significance only in the progression of fibers, when were compared the groups of animals with 4 and 16 days of healing, with the treatment of CMC 20 mg/mL associated with ADSCs. There was no statistical difference in the amount of collagen fibers in any group treated, when compared with control lesions (Sham). CMC: Sodium carboxymethylcellulose; ADSCs: Adipose-derived stem cells; Sham: Without treatment. A–C: Scale bar = 50 µm.