Widespread and enriched mutations across patient samples.

<p><b>(a)</b> Per-individual numbers, sorted from high to low, of somatic mutations falling in ZF position 9 in UCEC and COAD/READ, and in ZF position 11 in SKCM. <b>(b)</b> For each UCEC and COAD/READ tumor sample with a mutation in position 9 and each SKCM tumor sample with a mutation in position 11, the number of missense mutations at the specified ZF position is plotted against that patient’s total number of missense mutations. The Spearman’s rank correlation coefficient between these values is given in each panel. Samples with at least one R9I/H11Y mutation are shown in green/purple, respectively. <b>(c)</b> The histograms display the number of missense mutations in the positions of interest obtained in 10,000 trinucleotide context-preserving randomizations, while the red line shows the actual observed number. This permutation test reveals that the excess of missense mutations in positions 9 and 11 is statistically significant. <b>(d)</b> Box plots of the per-gene ratios of normalized nonsynonymous to synonymous mutation counts. ZF genes with at least one missense mutation at position 9 (UCEC, COAD/READ) or 11 (SKCM) tend to have higher ratios than other mutated genes (one-sided Mann-Whitney test <i>p</i>-values 2.4e-23, 1.1e-27, and 8.8e-28, respectively).