Additional file 5: of A gene regulatory network underlying the formation of pre-placodal ectoderm in Xenopus laevis

Figure S4. Six1 and Sox3 expression after overexpression of Dlx3 or Msx1. Transverse sections through neural plate or neural tube of Xenopus embryos after injection of Dlx3 (A) or Msx1 (B) mRNA and in situ hybridization for Six1. Sections are shown in brightfield (A1, B1) and in an overlay of red and UV fluorescent channels (A2, B2). LacZ (turquoise in A1 and B1) reveals the extent of mRNA injection in the neural plate (hatched outlines). Nuclei are stained by DAPI (blue). Sox3 immunopositive nuclei are shown in pink. Ectopic Six1 expression is confined to Dlx3- or Msx1-injected regions of the neural plate, which lack Sox3 immunoreactivity. Abbreviations: not, notochord, np: neural plate, nt: neural tube, PPE: preplacodal ectoderm, so: somite. Bar: 50Â Îźm (for all panels). (PDF 659 kb