Additional file 2: of A gene regulatory network underlying the formation of pre-placodal ectoderm in Xenopus laevis

Figure S1. Requirement of early ectodermal TFs for Eya1 expression in the PPE. Expression of PPE marker Eya1 in dorsal views of neural plate stage Xenopus embryos after injection of MOs blocking translation of early ectodermal TF genes. Anterior is to the bottom. Control side is shown on the left and injected side on the right (as indicated by blue LacZ staining). Reductions in the non-neural ectoderm (orange arrows) compared with the control side (orange arrowheads) are indicated. Green lines indicate broadening of the neural plate and lateral displacement of NPB markers on the injected side (bright green) versus control side (dark green). See Additional file 1: Table S3 for numbers. (PDF 254 kb