Additional file 3: of A gene regulatory network underlying the formation of pre-placodal ectoderm in Xenopus laevis

Figure S2. Cell autonomous requirements of Zic1 and Pax3 for PPE formation. Neural plates were orthotopically grafted from donor embryos to host embryos. A: Control grafts from GFP injected embryos into uninjected hosts. There is no effect on Six1 expression in the PPE (except for a slight decrease in 1/4 embryos). B: Grafting a neural plate from Pax3 MO injected embryo into uninjected hosts does not affect Six1 expression in the PPE (except for 1/10 cases). C: A neural plate graft from an uninjected embryo is unable to rescue deficits in Six1 expression in the PPE (arrow) of Pax3 MO injected embryos evident in 2/4 embryos. D: Grafting a neural plate from Zic1 MO injected embryo into uninjected hosts does not affect Six1 expression in the PPE (0/5). E: A neural plate graft from an uninjected embryo is unable to rescue deficits in Six1 expression in the PPE (arrow) of Zic1 MO injected embryos evident in 2/5 embryos. Asterisk indicates Six1 expression in graft. Arrowheads indicate the Six1 expression domain in the PPE on the control side. G: graft. (PDF 2727 kb