Expression of GluClα-EGFP in oocytes.

<p>(A) Cartoon design of EGFP fusion to the M3/M4 loop of GluClα. (B) Four days after injecting 50 ng of synthetic mRNA, receptor expression is visualized by EGFP fluorescence at the oocyte surface by confocal microscopy. (C) Detergent (C₁₂M) solubilization of oocytes and FSEC analysis on a Superose6 size exclusion column captures a monodisperse elution profile consistent with a pentameric assembly. Arrows indicate peak elution times for ferritin (440 kDa) and EGFP (27 kDa) standards. Receptor expression levels are sensitive to the total concentration of mRNA injected. FSEC traces were acquired from the same batch of oocytes. Absolute fluorescence intensities are shown.