Intracellular movement of functionalized FSs reveals active and polarized transport.

<p>(a) FS-PEG-BS69 injection into nls-GFP oocyte, 5 min post injection. White box highlights the region shown in a’, (Oo) indicates the oocyte nucleus, (A) anterior, (P) posterior. (a’) 20 time points (30 seconds (30 s) to 40 seconds (40 s)) projection from a 1 min movie taken 5 min after injection (2 frames/s). Arrows 1, 2 and 3 follow the path of actively transported particles; arrows 4 and 5 follow particles showing diffusion and static behavior, respectively. Movement characterization was made by MSD analysis. White line indicates the anterior boundary of the oocyte. (a’’) Series of 6 frames extracted from the movie. Yellow, green, blue, red and pink arrows show the movement of particles 1, 2, 3, 4, 5, respectively. Oocyte is on the left of the frame. (b) Graph showing the percentage of actively transported particles determined by MSD calculation. The numbers in the bars represent the total number of analyzed fluospheres for each condition. *, p<0.05; **, p<0.005; Chi-2 test of the different conditions versus FS-PEG-O-Me (5% critical value, 1 degree of freedom). (c) 30min after injection, FS-PEG-BS69 form aggregates around the oocyte nucleus. Some aggregates are closely associated to the nuclear envelope (arrows). (d) Graph showing the percentage of injected and fixed oocyte (45 min after injection) showing FSs aggregates (0 μm² 2) around the nucleus. The numbers represent the total number of analyzed oocyte for each condition. *, p<0.05; **, p<0.005; Chi-2 test of the different conditions versus FS-PEG-BS69 (5% critical value, 1 degree of freedom).