Additional file 17: of Assessing the benefits of horizontal gene transfer by laboratory evolution and genome sequencing

Excel file S1. List of de novo mutations in coding regions found in sequenced clones isolated from HPA evolved populations. The coordinates of mutations are given in coordinates of (i) the E. coli K12 reference genome for mutations in genes from the recipient K12 genome (sheet 1), (ii) the E. coli W genome in genes transferred from the E. coli W donor in Re c K W $$ \operatorname{Re}{\mathrm{c}}_{\mathrm{K}}^{\mathrm{W}} $$ clones (sheet 2), and (iii) the E. coli B genome in genes transferred from the E. coli W donor in Re c K B $$ \operatorname{Re}{\mathrm{c}}_{\mathrm{K}}^{\mathrm{B}} $$ clones (sheet 3) (In ' Re c Y X ’ $$ \operatorname{Re}{{\mathrm{c}}_{\mathrm{Y}}^{\mathrm{X}}}^{'} $$ ', X refers to the DNA donor identity, and Y refers to the recipient identity). Mutations are annotated according to the amino acid change they cause in the encoded polypeptide sequence. Specifically, the left first three-letter abbreviation denotes the original amino acid, the following number indicates its location, and the right abbreviation denotes the substituted amino acid. ‘fs’ denotes frameshift mutations, ‘del’ denotes a deletion, and ‘*’ denotes a stop-gain mutation. (XLSX 12 kb