Additional file 19: of Assessing the benefits of horizontal gene transfer by laboratory evolution and genome sequencing

Figure S12. Density of cells adapted to butyric acid and total cell density of the evolving replicate populations. (A) Total cell density (vertical axis, log scale) of evolving populations as a function of time (horizontal axis), as estimated by plating population samples on glucose-containing plates. Missing data indicates that no clones growing on the butyric acid plate were found or the replicate population had gone extinct (replicate populations not showed in the legend). (B) Fraction of butyric acid-adapted cells (Methods), measured every five days (~ 33 generations) during the butyric acid-adaptation experiment for each replicate population indicated in the color legend. ' Re c Y X $$ \operatorname{Re}{\mathrm{c}}_{\mathrm{Y}}^{\mathrm{X}} $$ ' denotes populations where an Y recipient recombines with an X donor, and replicate populations for any one such treatment are labeled with numbers in the legend. (C) Concentrations of glycerol (dark yellow) and butyric acid (pink) during the experiment. (EPS 121 kb