MGBG inhibited monocyte CD16 expression and BrdU incorporation.

<p>Isolated human monocytes were cultured in suspension with or without MGBG treatment. CD16 expression was measured by flow cytometry. (A). Monocytes differentiate spontaneously in culture. CD16 expression level increases in monocyte culture. n = 4, means and SEM. (B). MGBG inhibited CD16 expression in 1 day cultured monocytes in a dose-dependent manner. The average CD16 geometric mean fluorescence of 1 day untreated monocytes was measured at 108 units. n = 4, means and SEM. (C). MGBG inhibited BrdU incorporation in cultured monocytes. Isolated human monocytes were cultured for 3–6 days for BrdU incorporation using a FITC BrdU flow kit. The average percentage of BrdU incorporated untreated monocytes was measured at 4.7%. MGBG inhibited monocyte BrdU uptake in a dose-dependent manner. n = 5, means and SEM.