Pro-inflammatory activation of purified CD14++ monocytes during dietary intervention.

<p>Figures A to B show the purity analysis of CD14-magnetic-bead isolated human circulating monocytes. A, shows the percentage of CD14++ cells after CD14 bead purification. B, shows a CD14 versus CD16 color plot using cells after CD14 bead purification. Note that monocyte purified by this method are CD14++ monocytes (i.e., CD14++CD16− and CD14++CD16+, according to gating strategies described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060332#pone-0060332-g002" target="_blank"><b>Figure 2</b></a>). Figures C and D show intracellular ROS production in magnetic beads purified CD14++ monocytes by 2′, 7′-dichlorofluorescein (DCF) flow cytometry analysis (n = 6, one way repeated measures ANOVA).Figure E shows the related gene expression in magnetic-bead-purified CD14++ monocytes (n = 16, one way repeated measures ANOVA). “D3” to “D17” labeled in the x-axis indicates “day 3″ to “day 17″. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001. NF-κB indicates nuclear factor kappa B; MCP-1 indicates monocyte chemoattractant protein-1; RANTES indicates Regulated on Activation, Normal T Cell Expressed and Secreted (<i>or</i> CC chemokine ligand 5, CCL5); TGF-β indicates transforming growth factor β; Arg1 indicates arginase 1; MFI indicates median fluorescence intensity.