Effect of platelet-free conditioned medium on monocyte phenotype.

<p>Percentage of CD14⁺CD16⁺ monocytes (<b>A</b>) and MPA (<b>B</b>), following 48 h incubation of monocytes in medium alone (M), platelet-free conditioned medium either in the absence (M+PCM) or presence of anti-PSGL-1 blocking antibody (M+PCM+anti-PSGL-1), or platelets (M+P) (n = 3 for each). (<b>C</b>), Soluble P-selectin measured in the supernatant of monocytes cultured for 48 h under different experimental conditions as shown. (<b>D</b>), Percentage of CD14⁺CD16⁺ monocytes after 48 h exposure to different concentrations of soluble P-selectin. *** p<0.001 vs M.