MGBG inhibited polarization of monocytes into M1 and M2 macrophages.

<p>Isolated human monocytes were cultured to differentiate and induced to M1 or M2 macrophages using 50 ng/ml INF-γ and 1 μg/ml LPS or 50 ng/ml IL4 for 5–6 days with or without 0.4 μM MGBG. Cells were cultured and evaluated by flow cytometry at day 5 or 6. Mono: Baseline expression of cell surface markers in 4 hr cultured monocytes (% of all monocytes); Mac: Cultured in normal media -/+ 0.4 μM MGBG; M1: Cultured in M1 polarizing media -/+ 0.4 μM MGBG; M2: Cultured in M2 polarizing media -/+ 0.4 μM MGBG. MGBG inhibited monocyte differentiation in both M1 and M2 macrophages. n = 2.