Supplementary data. Schematic representations of the sequence coding for EhCP1 (Figure S1.) and the recombinant plasmid pQEhCP1 (Figure S2.). Analysis of recombinant EhCP1 purification (Figure S3.). (PDF 182 kb
Additional file 1: Table S1. Production of SFAs and UFAs in wild type Escherichia coli Rosetta overe...
Figure S2. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale gro...
SDS-PAGE analysis of rGST-COE expression at various induction temperatures. S, soluble fraction; I, ...
Figure S1. SDS-PAGE analysis of the purification of soluble, extracellular γ-CGTase expression by E....
Table S1. Summary of the purification of soluble extracellular γ-CGTase expressed by E. coli with an...
Additional file 1: Table S1. Primers used in this study. Table S2. Recombinant E. coli strains used ...
Bacterial strains and plasmids used in this study. Table S2. Oligo nucleotides used in this work. Ta...
Table S2. Comparison of parameters for recombinant Îł-CGTase production in shake flasks and a 3-L fe...
Scheme for de novo synthesising from oligonucleotides DNA fragments that encode the mlDL-Ds1, 2, 3 (...
Restriction enzyme digestion of plasmids containing two, four, and six expression cassettes. The pla...
Figure S2. Multiple alignments of the selected bacterial alkaline proteases. DHAP (GI:38373994); Sav...
Figure S1. Plasmid map of chromosomal knock-in vector in C. bescii for extracellular expression of E...
Expression and purification of the recombinant proteins rLbSCD6 (a) and rLbRBP42 (b). Analysis by Co...
Figure S1. Plasmids used for the production and secretion of rHtrA proteins. Plasmids pVE8126 (on th...
Table S1. Expression of antimicrobial peptides using E.coli and their subsequent release and purifi...
Additional file 1: Table S1. Production of SFAs and UFAs in wild type Escherichia coli Rosetta overe...
Figure S2. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale gro...
SDS-PAGE analysis of rGST-COE expression at various induction temperatures. S, soluble fraction; I, ...
Figure S1. SDS-PAGE analysis of the purification of soluble, extracellular γ-CGTase expression by E....
Table S1. Summary of the purification of soluble extracellular γ-CGTase expressed by E. coli with an...
Additional file 1: Table S1. Primers used in this study. Table S2. Recombinant E. coli strains used ...
Bacterial strains and plasmids used in this study. Table S2. Oligo nucleotides used in this work. Ta...
Table S2. Comparison of parameters for recombinant Îł-CGTase production in shake flasks and a 3-L fe...
Scheme for de novo synthesising from oligonucleotides DNA fragments that encode the mlDL-Ds1, 2, 3 (...
Restriction enzyme digestion of plasmids containing two, four, and six expression cassettes. The pla...
Figure S2. Multiple alignments of the selected bacterial alkaline proteases. DHAP (GI:38373994); Sav...
Figure S1. Plasmid map of chromosomal knock-in vector in C. bescii for extracellular expression of E...
Expression and purification of the recombinant proteins rLbSCD6 (a) and rLbRBP42 (b). Analysis by Co...
Figure S1. Plasmids used for the production and secretion of rHtrA proteins. Plasmids pVE8126 (on th...
Table S1. Expression of antimicrobial peptides using E.coli and their subsequent release and purifi...
Additional file 1: Table S1. Production of SFAs and UFAs in wild type Escherichia coli Rosetta overe...
Figure S2. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale gro...
SDS-PAGE analysis of rGST-COE expression at various induction temperatures. S, soluble fraction; I, ...