Figure S1. SDS-PAGE analysis of the purification of soluble, extracellular γ-CGTase expression by E. coli with and without β-cyclodextrin added. (DOCX 202 kb
Figure S3. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale gro...
Expression and purification of rCsGSTo1 and 2. a The full-length ORFs of CsGSTo1 and 2 were transfor...
Figure S2. Growth curves for E. coli BW25113, PM2405, and PM2405A with various concentrations of ara...
Table S1. Summary of the purification of soluble extracellular γ-CGTase expressed by E. coli with an...
Table S2. Comparison of parameters for recombinant Îł-CGTase production in shake flasks and a 3-L fe...
Supplementary data. Schematic representations of the sequence coding for EhCP1 (Figure S1.) and the ...
The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully...
Bacterial strains and plasmids used in this study. Table S2. Oligo nucleotides used in this work. Ta...
SDS-PAGE analysis of rGST-COE expression at various induction temperatures. S, soluble fraction; I, ...
Restriction enzyme digestion of plasmids containing two, four, and six expression cassettes. The pla...
Overnight growth of wild type E. coli cells carrying empty or MNase-expressing vector on LB-agar-amp...
Table S1. Oligonucleotides used in this study. Figure S1 Plasmid maps of pVWEx1-nagE [A] and pEKEx3-...
Table S1. Bacterial strains and plasmids used in this work. Table S2. Primers used in this work. Fig...
Detailed scheme of isolating mlDL isoforms from inclusion bodies and their optimisation of renaturat...
The cloning of a polymerase chain reaction (PCR) gene fragment from Bacillus sp. NR5 UPM isolated fr...
Figure S3. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale gro...
Expression and purification of rCsGSTo1 and 2. a The full-length ORFs of CsGSTo1 and 2 were transfor...
Figure S2. Growth curves for E. coli BW25113, PM2405, and PM2405A with various concentrations of ara...
Table S1. Summary of the purification of soluble extracellular γ-CGTase expressed by E. coli with an...
Table S2. Comparison of parameters for recombinant Îł-CGTase production in shake flasks and a 3-L fe...
Supplementary data. Schematic representations of the sequence coding for EhCP1 (Figure S1.) and the ...
The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully...
Bacterial strains and plasmids used in this study. Table S2. Oligo nucleotides used in this work. Ta...
SDS-PAGE analysis of rGST-COE expression at various induction temperatures. S, soluble fraction; I, ...
Restriction enzyme digestion of plasmids containing two, four, and six expression cassettes. The pla...
Overnight growth of wild type E. coli cells carrying empty or MNase-expressing vector on LB-agar-amp...
Table S1. Oligonucleotides used in this study. Figure S1 Plasmid maps of pVWEx1-nagE [A] and pEKEx3-...
Table S1. Bacterial strains and plasmids used in this work. Table S2. Primers used in this work. Fig...
Detailed scheme of isolating mlDL isoforms from inclusion bodies and their optimisation of renaturat...
The cloning of a polymerase chain reaction (PCR) gene fragment from Bacillus sp. NR5 UPM isolated fr...
Figure S3. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale gro...
Expression and purification of rCsGSTo1 and 2. a The full-length ORFs of CsGSTo1 and 2 were transfor...
Figure S2. Growth curves for E. coli BW25113, PM2405, and PM2405A with various concentrations of ara...
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