Additional file 3: of A comparison of the enzymatic properties of three recombinant isoforms of thrombolytic and antibacterial protein—Destabilase-Lysozyme from medicinal leech

Scheme for de novo synthesising from oligonucleotides DNA fragments that encode the mlDL-Ds1, 2, 3 (optymized for E. coli ) and map of plasmid, encoding mlDL. Figure S2. Fragment of pET15MCS plasmid containing multicloning sites. PT7—promoter of late genes of bacteriophage T7; RBS—ribosome binding site; ATG—start codon; MCS—multiple cloning site; stop—stop codon; 6His—fragment encoding six histidines; tromb—fragment encoding the recognition site of thrombin. Figure S3. A map of the plasmid pET15/Dest. PT7—promoter of bacteriophage T7 late genes; dest—fragment encoding the mlDL isoforms; 6His—region encoding the hexa-histidine motive; stop—translation terminator; term—a transcription terminator; bla—beta-lactamase gene; pBR322ori—an origin of replication of plasmid pBR322; lacI—gene lacI of E. coli; tromb—sequence encoding the recognition site of thrombin. (PDF 119 kb