Muramidase activity of mlDL isoforms. Effects of pH (a) and ionic strength (b, c) on the muramidase activity of mlDL-Ds1. Muramidase activity of mlDL-Ds2. Effects of pH (d) and ionic strength (e, f) on the muramidase activity of mlDL-Ds2. The activity (in units) was calculated relative to the reference enzyme HEWL according to formula (1). (n = 5). (PDF 106 kb
Additional file 7: Figure S5. Purification and specific activity of MAN330. Transformants containing...
Polynucleotide kinase activity assay. PNK reaction mixtures of A) T4 PNK, B) MmCNPase C) MmCNP_N and...
Additional file 1: Figure S1. Effect of pH (a) and temperature (b) on the activity of TtXyn30A again...
Isopeptidase activity of mlDL isoforms. The effect of pH (a) and ionic strength (b) at pH 5.5 on the...
Comparison of amino acid sequences of mlDL isoforms. Catalytic residuesresponsible for muramidase ac...
CD spectra of mlDL isoforms at different pH conditions (2.2, 3.2, 6.5). (PDF 84Â kb
Absorbance at 405 nm of M. lysodeikticus suspension during mlDL-Ds3 treatment at 25 °C for 20 min in...
Detailed scheme of isolating mlDL isoforms from inclusion bodies and their optimisation of renaturat...
Scheme for de novo synthesising from oligonucleotides DNA fragments that encode the mlDL-Ds1, 2, 3 (...
Additional file 3: Table S1. Experimental protocol of ion-exchange chromatography. Table S2. Experim...
Figure S4. Expression and hemolytic activity of P450139. (a) Coomassie-stained SDS-PAGE showing hete...
Interaction of Mus81-Mms4 with PCNA and PCNA-dependent stimulation of Mus81-Mms4 activity. (A) Purif...
Table S1. Saturation mutagenesis primers. Figure S1. Purification of wild-type and mutants. M: Marke...
Additional file 1: Table S1. Protein concentration (determined by BCA assay) of the secretomes of fi...
Additional file 1: Figure S1. Effects of a pH and b temperature on the activity of the wild-type (cl...
Additional file 7: Figure S5. Purification and specific activity of MAN330. Transformants containing...
Polynucleotide kinase activity assay. PNK reaction mixtures of A) T4 PNK, B) MmCNPase C) MmCNP_N and...
Additional file 1: Figure S1. Effect of pH (a) and temperature (b) on the activity of TtXyn30A again...
Isopeptidase activity of mlDL isoforms. The effect of pH (a) and ionic strength (b) at pH 5.5 on the...
Comparison of amino acid sequences of mlDL isoforms. Catalytic residuesresponsible for muramidase ac...
CD spectra of mlDL isoforms at different pH conditions (2.2, 3.2, 6.5). (PDF 84Â kb
Absorbance at 405 nm of M. lysodeikticus suspension during mlDL-Ds3 treatment at 25 °C for 20 min in...
Detailed scheme of isolating mlDL isoforms from inclusion bodies and their optimisation of renaturat...
Scheme for de novo synthesising from oligonucleotides DNA fragments that encode the mlDL-Ds1, 2, 3 (...
Additional file 3: Table S1. Experimental protocol of ion-exchange chromatography. Table S2. Experim...
Figure S4. Expression and hemolytic activity of P450139. (a) Coomassie-stained SDS-PAGE showing hete...
Interaction of Mus81-Mms4 with PCNA and PCNA-dependent stimulation of Mus81-Mms4 activity. (A) Purif...
Table S1. Saturation mutagenesis primers. Figure S1. Purification of wild-type and mutants. M: Marke...
Additional file 1: Table S1. Protein concentration (determined by BCA assay) of the secretomes of fi...
Additional file 1: Figure S1. Effects of a pH and b temperature on the activity of the wild-type (cl...
Additional file 7: Figure S5. Purification and specific activity of MAN330. Transformants containing...
Polynucleotide kinase activity assay. PNK reaction mixtures of A) T4 PNK, B) MmCNPase C) MmCNP_N and...
Additional file 1: Figure S1. Effect of pH (a) and temperature (b) on the activity of TtXyn30A again...