Additional file 1 of Functional analysis of the dehydratase domains of the PUFA synthase from Emiliania huxleyi in Escherichia coli and Arabidopsis thaliana

Additional file 1: Table S1. Production of SFAs and UFAs in wild type Escherichia coli Rosetta overexpressing EhDH domains. Table S2. Production of SFAs and UFAs in wild-type Escherichia coli Rosetta overexpressing the mutated EhDH domains. Table S3. Primers used for DH domain amplifications. Table S4. Primers used for site-directed mutagenesis of DH domains. Table S5. Primers used for plant experiment. Figure S1. Expression of recombinant proteins when induced with 0.2 mM IPTG in Escherichia coli (E. coli). a SDS-PAGE and WB analysis of the extracellular secretion from E. coli Rosetta strains overexpressing EhDH domains. b WB analysis of the extracellular secretion from E. coli Rosetta strains expressing site-directed mutagenized EhDH domains. SDS-PAGE, the sodium dodecyl sulphate-polyacrylamide gel electrophoresis; WB, western blotting. The marker used in this experiment is 180 kDa Prestained Protein Marker (180, 130, 100, 70, 55, 40, 35, 25, 15 kDa); 1, pET32a-EhDH1; 2, pET32a-EhDH1-M; 3, pET32a-EhDH2; 4, pET32a-EhDH2-M; 5, pET32a-EhDH1-DH2; 6, pET32a-EhDH1-M-DH2; 7, pET32a-EhDH1-DH2-M; 8 pET32a-TcDH1-DH2; 9 pET32a (negative control). Figure S2. Schematic representation of the T-DNA region of the binary plasmid vector expressing tandem EhDH domains