Additional file 1: of Classification of barley U-box E3 ligases and their expression patterns in response to drought and pathogen stresses

Figure S1. Domain structures and phylogenetic analysis of Class II genes in Arabidopsis. A. Phylogenetic analysis of 27 Class II PUB genes in barley. Brown dot, subclass a; blue dot, subclass b. B. Full-length amino-acid sequences of ARM repeat domain were aligned using the Clustal X2 software. The tree was constructed by neighbor-joining method after bootstrap analysis for 1000 replicates [1]. C. Domain structures of 27 Class II PUB genes. Green box, U-box domain; skyblue box, ARM repeat domain; blue box, Heat domain. Figure S2. Domain structures and phylogenetic analysis of Class II genes in rice. A. Phylogenetic analysis of 25 Class II PUB genes in barley. Brown dot, subclass a; blue dot, subclass b. B. Full-length amino-acid sequences of ARM repeat domain were aligned using the Clustal X2 software. The tree was constructed by neighbor-joining method after bootstrap analysis for 1000 replicates [1]. C. Domain structures of 25 Class II PUB genes. Green box, U-box domain; skyblue box, ARM repeat domain; blue box, Heat domain. Figure S3. Class III genes in Arabidopsis and rice, those were converted to new classes, Class II and Class V. Figure S4. Schematic domain structures of Class IV genes in Arabidopsis, rice and barley. Figure S5. Phylogenetic analysis of Class IV PUB genes in Arabidopsis, rice and barely. Figure S6. Domain structures of ClassVI PUB genes in Barley. Figure S7. The expression profiles of HvPUB genes in response to drought stress. A. HvPUB genes are up-regulated by drought stress. B. HvPUB genes are down-regulated by drought stress. Figure S8. The expression profiles of HvPUB genes in response to biotic stress. A. HvPUB genes are up-regulated by biotic stress. B. HvPUB genes are down-regulated by biotic stress. (DOCX 3262 kb