Additional file 1 of Polyhydroxybutyrate production by recombinant Escherichia coli based on genes related to synthesis pathway of PHB from Massilia sp. UMI-21

Additional file 1: Table S1. PHB-producing strains in the genus Massilia. Table S2. Primers used in recombinant plasmid construction. Fig. S1. Phylogenetic tree of PHA-related proteins from Massilia sp. UMI-21 and related taxa. (A) PhaA. (B) PhaB. (C) PhaC. The GenBank accession numbers were shown in the parentheses. Green denotes Class III and IV PhaC; orange denotes Class I and II PhaC. Fig. S2. Results of phaA gene and amino acid (AA) sequence alignment between Ralstonia eutropha H16 and Massilia sp. UMI-21. (A) Alignment of DNA sequences. (B) Alignment of AA sequences. Fig. S3. Results of phaB gene and amino acid (AA) sequence alignment between Ralstonia eutropha H16 and Massilia sp. UMI-21. (A) Alignment of DNA sequences. (B) Alignment of AA sequences. Fig. S4. Results of phaC gene and amino acid (AA) sequence alignment between Ralstonia eutropha H16 and Massilia sp. UMI-21. (A) Alignment of DNA sequences. (B) Alignment of AA sequences. Fig. S5. Relative expression of phaA2 (A), phaB1 (B) and phaC1 (C) genes in wild E. coli DH5α strain and the recombinant E. coli DH5α-pETDuet1-phaA2phaB1-phaC1 and DH5α-pETDuet1-phaA2phaB1-phaC1-vgb strains by qRT-PCR. 16S rRNA serves as the internal contro