Level of apoptosis.

<p>Representative images of TUNEL(A) and Cleaved Caspase-3(B) immunohistochemistry showing a lower grade of apoptosis in RGZ vs Control group at 1hr and 6hr post-reperfusion. Significant lower TUNEL-staining was found in the RGZ vs CTRL group at 1hr (2.46±0.49 vs 6.90±0.85%; p = 0.001) and 6hrs (26.40±2.93 vs 50.13±8.29%; p = 0.048) post-reperfusion by image analysis(C). Cleaved caspase-3 immunohistochemistry also demonstrated less staining in the RGZ group vs control group, the difference was assessed by image software analysis (D). Abbreviations: TUNEL-Terminal deoxynucleotidyl transferase(TdT) dUTP Nick-End-Labeling, RGZ-Rosiglitazone, Ctrl-Control. Five experiments (n = 5) per group per time point were performed. Results are shown as mean ± SEM, Mann-Whitney-U-test.