TUNEL assay of primary RGCs treated with endothelins.

<p>Primary RGCs were either untreated or treated with ET-1 (10 nM), ET-1 (100 nM) or ET-3 (100 nM) and TUNEL assays were carried out to detect apoptosis. The left vertical panel (TUNEL) indicates fluorescent images from cells incorporating fluorescinated dUTP indicative of apoptosis. The right vertical panel (DAPI) shows stained nuclei using DAPI. Scale bar indicates 200 µm.