RLIPC reduces pulmonary apoptosis post I/R.

<p><b><i>A</i></b>. Terminal transferase dUTP nick end labeling (TUNEL) technique was applied for the analysis of pulmonary apoptosis. <b><i>Left</i></b>, TUNEL-positive nuclei in the left lungs obtained from sham-operated (sham), liver conditioned (RLIPC) and control (CON) rats after pulmonary reperfusion injury were stained green (TUNEL) and nuclei were stained red (DAPI). Scale bars, 20μm. <b><i>Right</i></b>, Bar graph showing the TUNEL-positive nuclei expressed as a percentage of total nuclei in the left lung sections; <i>n</i> = 4–6. All data were expressed as mean ± SEM. ***P<0.001 compared with sham-operated lungs, ^##P<0.01, ^###P<0.001, compared with CON (by One-way ANOVA). <b><i>B</i></b>. Representative Western blots (<b><i>left</i></b>) and quantification (<b><i>right</i></b>) of cleaved caspase-3 protein band density (normalized to GAPDH) in sham, CON, and liver-conditioned rat left lungs are shown; <i>n</i> = 5. <i>**P<0</i>.<i>05</i>, compared with sham-operated rats; ^<i>#</i><i>P<0</i>.<i>05</i>, ^<i>##</i><i>P<0</i>.<i>01</i>, compared with CON (by One-way ANOVA).