Representative images of Ki-67, TUNEL, Caspase-3 (Active) and PARP-1 (Cleaved 85) at the end of experiments after combination and control treatments.

<p>A. Very low Ki-67 expression is seen in the combination test; reduced Ki-67 expression in the combination control and MAb C595 control while high Ki-67 expression is seen in the vehicle control (<i>P</i><0.05). B. Obvious TUNEL-positive cells are shown in combination test; some TUNEL-positive cells are shown in the combination control and MAb C595 control while no TUNEL-positive cells are seen in the vehicle control (<i>P</i><0.05). C. High caspase-3 (active) expression is shown in combination test; low caspase-3 (active) expression is shown in combination control and MAb C595 control while negative caspase-3 (active) expression is shown in vehicle control (<i>P</i><0.05). D. High PARP-1 (cleaved p85) expression is shown in combination test; low PARP-1 (cleaved p85) expression is shown in combination control and MAb C595 control while negative PARP-1 (cleaved p85) expression is shown in vehicle control (<i>P</i><0.05). The brown color indicates nuclear staining in Ki-67, caspase-3 (active) and PARP-1 (cleaved p85), while blue indicates nuclear staining with hematoxylin. In the TUNEL assay, the brown color indicates nuclear chromatin condensation and fragmentation with methylgreen nuclear staining. Magnification ×20 in B; Magnification ×40 in A, C, D. Representative images are shown.