Additional file 5: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system
- Cuicui Lyu (5072255)
- Jun Shen (15131)
- Rui Wang (52434)
- Haihui Gu (664421)
- Jianping Zhang (161794)
- Feng Xue (238913)
- Xiaofan Liu (141518)
- Wei Liu (20030)
- Rongfeng Fu (5072252)
- Liyan Zhang (322168)
- Huiyuan Li (1321050)
- Xiaobing Zhang (12137)
- Tao Cheng (1235148)
- Renchi Yang (5072249)
- Lei Zhang (38117)
Publication date
April 2018
DOIAbstract
Figure S2 showing characterization of iPSC colony 5. a Karyotype of iPSC colony 5 was normal. b qRT-PCR analysis showed expression of OCT4, SOX2, and NANOG of iPSC colony 5. PBMNCs of patient used as negative control, H1 embryonic stem cells used as positive control. c Immunofluorescence staining showed expression of TRA-1-60, SSEA4, OCT4, and NANOG. d Sections of teratomas stained with H&E (endoderm: pancreas; mesoderm: muscle; ectoderm: nerve fibers). All scale bars represent 100 Îźm. (DOCX 1261 kb
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