Figure S1. showing sequencing results of parental and inserted iPSCs. a Parental iPSCs have the known F9 gene mutation c.676C > T, p.Arg226Trp. b Inserted iPSCs (colony 5) have a heterozygous mutation of c.676C > T, p.Arg226Trp. (DOCX 393 kb
Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...
Figure S1. Strategy for targeting the promoter and IVS1–110 mutation of the HBB gene. A) The promote...
Oligos used to introduce sgRNA into px330. Each pair of forward and reverse primers were annealing a...
Table S3 presenting plasmids used for transfection of HEK293T cells and iPSCs. (DOCX 14 kb
Table S1. presenting antibodies used for immunofluorescence staining and flow cytometry analysis. (D...
Figure S2 showing characterization of iPSC colony 5. a Karyotype of iPSC colony 5 was normal. b qRT-...
Figure S3. showing off-target effects detection in successful inserted iPSCs. Using Cas-OFFinder, 17...
Table S2. presenting primers used for characterization of hepatocytic functions. (DOCX 14 kb
Figure S4. showing characterization of hepatocytic functions. Differentiated cells had functions of ...
Showing transfection efficiency of PX458 in the Eβ-iPSC2 cells. (a) Phase contrast and fluorescent i...
Showing representative karyotypes of the corrected C22, C134, C137 and C258 cells, which exhibited n...
Showing gene expression profile of the differentiated cells. (a) qRT-PCR analysis of hematopoietic ...
Showing characterization of iPSCs derived from skin fibroblasts of a patient with hemoglobin E/beta-...
Figure S2. CRISPR-Cas9 targeting strategy and editing efficiency. (a) Schematic of exon 1 of human P...
is Figure S1 showing cellular characteristics and HPC/HSC reprogramming. A Identification of HPC/HSC...
Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...
Figure S1. Strategy for targeting the promoter and IVS1–110 mutation of the HBB gene. A) The promote...
Oligos used to introduce sgRNA into px330. Each pair of forward and reverse primers were annealing a...
Table S3 presenting plasmids used for transfection of HEK293T cells and iPSCs. (DOCX 14 kb
Table S1. presenting antibodies used for immunofluorescence staining and flow cytometry analysis. (D...
Figure S2 showing characterization of iPSC colony 5. a Karyotype of iPSC colony 5 was normal. b qRT-...
Figure S3. showing off-target effects detection in successful inserted iPSCs. Using Cas-OFFinder, 17...
Table S2. presenting primers used for characterization of hepatocytic functions. (DOCX 14 kb
Figure S4. showing characterization of hepatocytic functions. Differentiated cells had functions of ...
Showing transfection efficiency of PX458 in the Eβ-iPSC2 cells. (a) Phase contrast and fluorescent i...
Showing representative karyotypes of the corrected C22, C134, C137 and C258 cells, which exhibited n...
Showing gene expression profile of the differentiated cells. (a) qRT-PCR analysis of hematopoietic ...
Showing characterization of iPSCs derived from skin fibroblasts of a patient with hemoglobin E/beta-...
Figure S2. CRISPR-Cas9 targeting strategy and editing efficiency. (a) Schematic of exon 1 of human P...
is Figure S1 showing cellular characteristics and HPC/HSC reprogramming. A Identification of HPC/HSC...
Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...
Figure S1. Strategy for targeting the promoter and IVS1–110 mutation of the HBB gene. A) The promote...
Oligos used to introduce sgRNA into px330. Each pair of forward and reverse primers were annealing a...