Additional file 4: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Additional file 4: of Highly efficient and expedited hepatic differentiation from human pluripotent stem cells by pure small-molecule cocktails

Cong Du (2702083)
Yuan Feng (213823)
Dongbo Qiu (4927330)
Yan Xu (14594)
Mao Pang (710425)
Nan Cai (4927327)
Andy Xiang (3471293)
Qi Zhang (28502)

March 2018

Figure S3. qRT-PCR for pluripotency genes using RNA lysates from hESC-H7 at the endpoint of stage I....

Additional file 5: Figure S4. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing gene expression profile ofÂ the differentiated cells. (a) qRT-PCR analysis of hematopoietic ...

Additional file 2: of Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Xiaofeng Liu (35910)
Min Wang (21070)
Yufeng Qin (290016)
Xuan Shi (739853)
Peiqing Cong (134479)
Yaosheng Chen (134488)
Zuyong He (134485)

October 2018

Figure S1. Targeted integration of single donor plasmid at the CCR5 locus in HEK293T cells through s...

Additional file 1: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Table S1. presenting antibodies used for immunofluorescence staining and flow cytometry analysis. (D...

Additional file 2: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Table S2. presenting primers used for characterization of hepatocytic functions. (DOCX 14 kb

Additional file 3: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S1. showing sequencing results of parental and inserted iPSCs. a Parental iPSCs have the know...

Additional file 5: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S2 showing characterization of iPSC colony 5. a Karyotype of iPSC colony 5 was normal. b qRT-...

Additional file 7: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S4. showing characterization of hepatocytic functions. Differentiated cells had functions of ...

Additional file 6: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S3. showing off-target effects detection in successful inserted iPSCs. Using Cas-OFFinder, 17...

Generation of a gene edited hemophilia A patient-derived iPSC cell line, YCMi001-B-1, by targeted insertion of coagulation factor FVIII using CRISPR/Cas9

김동욱

October 2020

Hemophilia A is an ideal target for cell or gene therapy because a mild increase in coagulation fact...

Additional file 4: Figure S3. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing representative karyotypes of the corrected C22, C134, C137 and C258 cells, which exhibited n...

Additional file 3: Figure S2. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing transfection efficiency of PX458 in the Eβ-iPSC2 cells. (a) Phase contrast and fluorescent i...

Additional file 1: of Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Xiaofeng Liu (35910)
Min Wang (21070)
Yufeng Qin (290016)
Xuan Shi (739853)
Peiqing Cong (134479)
Yaosheng Chen (134488)
Zuyong He (134485)

October 2018

Table S1. Target sequences within the CCR5 gene of engineered nucleases. Table S2. Primers used for ...

Additional file 3: of Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Xiaofeng Liu (35910)
Min Wang (21070)
Yufeng Qin (290016)
Xuan Shi (739853)
Peiqing Cong (134479)
Yaosheng Chen (134488)
Zuyong He (134485)

October 2018

Figure S2. Targeted integration of multiple donor plasmids at the CCR5 locus in HEK293T cells throug...

Additional file 1: of Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Chenzi Zhang (6163493)
Xiangjun He (6163496)
Yvonne Kwok (6163499)
Feng Wang (44414)
Junyi Xue (1533826)
Hui Zhao (7395)
Kin Suen (6163502)
Chi Wang (131921)
Jianwei Ren (438694)
George Chen (179705)
Paul Lai (6163505)
Jiangchao Li (290762)
Yin Xia (307747)
Andrew Chan (352984)
Wai-Yee Chan (18789)
Bo Feng (141991)

December 2018

Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...

Additional file 4: of Highly efficient and expedited hepatic differentiation from human pluripotent stem cells by pure small-molecule cocktails

Cong Du (2702083)
Yuan Feng (213823)
Dongbo Qiu (4927330)
Yan Xu (14594)
Mao Pang (710425)
Nan Cai (4927327)
Andy Xiang (3471293)
Qi Zhang (28502)

March 2018

Figure S3. qRT-PCR for pluripotency genes using RNA lysates from hESC-H7 at the endpoint of stage I....

Additional file 5: Figure S4. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing gene expression profile ofÂ the differentiated cells. (a) qRT-PCR analysis of hematopoietic ...

Additional file 2: of Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Xiaofeng Liu (35910)
Min Wang (21070)
Yufeng Qin (290016)
Xuan Shi (739853)
Peiqing Cong (134479)
Yaosheng Chen (134488)
Zuyong He (134485)

October 2018

Figure S1. Targeted integration of single donor plasmid at the CCR5 locus in HEK293T cells through s...

Additional file 1: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Table S1. presenting antibodies used for immunofluorescence staining and flow cytometry analysis. (D...

Additional file 2: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Table S2. presenting primers used for characterization of hepatocytic functions. (DOCX 14 kb

Additional file 3: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S1. showing sequencing results of parental and inserted iPSCs. a Parental iPSCs have the know...

Additional file 5: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S2 showing characterization of iPSC colony 5. a Karyotype of iPSC colony 5 was normal. b qRT-...

Additional file 7: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S4. showing characterization of hepatocytic functions. Differentiated cells had functions of ...

Additional file 6: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Cuicui Lyu (5072255)
Jun Shen (15131)
Rui Wang (52434)
Haihui Gu (664421)
Jianping Zhang (161794)
Feng Xue (238913)
Xiaofan Liu (141518)
Wei Liu (20030)
Rongfeng Fu (5072252)
Liyan Zhang (322168)
Huiyuan Li (1321050)
Xiaobing Zhang (12137)
Tao Cheng (1235148)
Renchi Yang (5072249)
Lei Zhang (38117)

April 2018

Figure S3. showing off-target effects detection in successful inserted iPSCs. Using Cas-OFFinder, 17...

Generation of a gene edited hemophilia A patient-derived iPSC cell line, YCMi001-B-1, by targeted insertion of coagulation factor FVIII using CRISPR/Cas9

김동욱

October 2020

Hemophilia A is an ideal target for cell or gene therapy because a mild increase in coagulation fact...

Additional file 4: Figure S3. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing representative karyotypes of the corrected C22, C134, C137 and C258 cells, which exhibited n...

Additional file 3: Figure S2. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing transfection efficiency of PX458 in the Eβ-iPSC2 cells. (a) Phase contrast and fluorescent i...

Additional file 1: of Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Xiaofeng Liu (35910)
Min Wang (21070)
Yufeng Qin (290016)
Xuan Shi (739853)
Peiqing Cong (134479)
Yaosheng Chen (134488)
Zuyong He (134485)

October 2018

Table S1. Target sequences within the CCR5 gene of engineered nucleases. Table S2. Primers used for ...

Additional file 3: of Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Xiaofeng Liu (35910)
Min Wang (21070)
Yufeng Qin (290016)
Xuan Shi (739853)
Peiqing Cong (134479)
Yaosheng Chen (134488)
Zuyong He (134485)

October 2018

Figure S2. Targeted integration of multiple donor plasmids at the CCR5 locus in HEK293T cells throug...

Additional file 1: of Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Chenzi Zhang (6163493)
Xiangjun He (6163496)
Yvonne Kwok (6163499)
Feng Wang (44414)
Junyi Xue (1533826)
Hui Zhao (7395)
Kin Suen (6163502)
Chi Wang (131921)
Jianwei Ren (438694)
George Chen (179705)
Paul Lai (6163505)
Jiangchao Li (290762)
Yin Xia (307747)
Andrew Chan (352984)
Wai-Yee Chan (18789)
Bo Feng (141991)

December 2018

Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...

Additional file 4: of Highly efficient and expedited hepatic differentiation from human pluripotent stem cells by pure small-molecule cocktails

Cong Du (2702083)
Yuan Feng (213823)
Dongbo Qiu (4927330)
Yan Xu (14594)
Mao Pang (710425)
Nan Cai (4927327)
Andy Xiang (3471293)
Qi Zhang (28502)

March 2018

Figure S3. qRT-PCR for pluripotency genes using RNA lysates from hESC-H7 at the endpoint of stage I....

Additional file 5: Figure S4. of One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

Methichit Wattanapanitch (627973)
Nattaya Damkham (4889524)
Ponthip Potirat (4889521)
Kongtana Trakarnsanga (597732)
Montira Janan (4889527)
Yaowalak U-pratya (627977)
Pakpoom Kheolamai (627979)
Nuttha Klincumhom (627974)
Surapol Issaragrisil (627981)

February 2018

Showing gene expression profile ofÂ the differentiated cells. (a) qRT-PCR analysis of hematopoietic ...

Additional file 2: of Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Xiaofeng Liu (35910)
Min Wang (21070)
Yufeng Qin (290016)
Xuan Shi (739853)
Peiqing Cong (134479)
Yaosheng Chen (134488)
Zuyong He (134485)

October 2018

Figure S1. Targeted integration of single donor plasmid at the CCR5 locus in HEK293T cells through s...

Additional file 4: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Abstract

Extracted data

Additional file 4: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system

Abstract

Extracted data

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