Additional file 3: of High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation

Figure S2. Derivation of hESCs from diseased and frozen–thawed embryos using MTP. (A) ICM clump isolated from a frozen–thawed aneuploid 3PN embryo and cultured on Matrigel-coated plate. After culture for 3 days, most TE cells degenerated (arrowhead) while remaining ICM cells were transferred onto fresh HFFs and expanded further to generate hESCs (Si3PN). (B) ICM clump isolated from Hb Bart’s hydrops fetalis embryos and cultured on Matrigel-coated plate. Degenerating TE cells (arrowhead) observed while remaining ICM cells were transferred onto fresh HFFs and expanded further to generate hESCs (SiAtha1). (C) Karyotyping results show Si3PN exhibited a triploid (69, XXY) karyotype while (D) SiAtha1 exhibited a normal diploid karyotype (46, XY). Scale bar: 50 μm. (JPG 1215 kb