Development of an improved TAC125 strain for recombinant protein secretion at low temperature-5

), and middle (32 h) (lane 2) exponential phase. In this experiment the zymographic developing time was 18 h, a condition that assures the detection of all proteases contained in the sample. Panel B: Protease zymography of a TAC125 wild type culture supernatant, collected at 24 h, untreated (NONE) and treated with protease inhibitors (10 mM EDTA, 10 mM PMSF, and the combination of the two inhibitors both at 10 mM final concentration). In this experiment a zymographic developing time of 12 h was chosen, this condition allows a clearer visualization and comparison of the proteases contained in the different samples. Panel C: Protease zymography of TAC125 mutant culture supernatants collected at early (24 h) (lane 1), and middle (32 h) (lane 2) exponential phase, the zymographic developing time was 18 h. Panel D: Protease zymography of a TAC125 mutant culture supernatant, collected at 24 h, untreated (NONE) and treated with protease inhibitors, the zymographic developing time was 12 h).<p><b>Copyright information:</b></p><p>Taken from "Development of an improved TAC125 strain for recombinant protein secretion at low temperature"</p><p>http://www.microbialcellfactories.com/content/7/1/2</p><p>Microbial Cell Factories 2008;7():2-2.</p><p>Published online 7 Feb 2008</p><p>PMCID:PMC2275215.</p><p>