MAB02 zymogram activity of protease deletion strain supernatants.

<p>White regions on the blue stained gel indicate an area of protease activity against MAB02. The major activity occurs between 65–90 kDa and a smaller protease activity occurs at 28 kDa. Culture supernatant samples from day 7 were diluted 1:2 in sodium citrate buffer (50 mM, pH 5.5) and 30 μl was loaded into a 12% zymogram SDS PAGE gel containing MAB02. The M124 parental strain, M181 (Δ<i>pep1</i>), M219 (Δ<i>pep1</i>Δ<i>tsp1</i>), and M277 (Δ<i>pep1</i>Δ<i>tsp1</i>Δ<i>slp1</i>) supernatants were assayed.