Continuous enzymatic assay for proDer p 3 maturation by Der p 1.
- Marie-Eve Dumez (461300)
- Julie Herman (461301)
- Vincenzo Campisi (461302)
- Ahlem Bouaziz (461303)
- Frédéric Rosu (31231)
- André Luxen (36813)
- Isabel Vandenberghe (461304)
- Edwin de Pauw (1718794)
- Jean-Marie Frère (461305)
- André Matagne (330049)
- Andy Chevigné (461306)
- Moreno Galleni (330047)
Publication date
September 2013
DOIAbstract
<p><b>A</b>: proDer p 3 (black) and P2A (gray), <b>B</b>: P5A and <b>C</b>: P8A (black) and P-A (gray) mutants. Substrate hydrolysis (10 µM IEGR-MCA) versus time curves showing the activation of 12.5 nM zymogens by Der p 1 with the indicated concentrations in 50 mM polybuffer 2 at 37°C.</p
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