BoNT/A intoxication is prevented in mice by co-administration of a pool of epitopically tagged VHHs and an anti-tag mAb.

<p>Symptoms of intoxication and time to death were monitored following administration of the indicated dose of BoNT/A in groups of five mice co-administered with pools of anti-BoNT/A VHHs+/−clearing Ab. The time to death is plotted as % survival as a function of time following administration of the indicated dose of toxin (LD₅₀). The pool of ciA-VHHs or control (no agents) that was administered to the mice is indicated by arrows. The presence or absence (dashed lines) of the anti-E-tag clearing Ab (αE) is also indicated. (<b>A</b>) Protection from BoNT/A lethality by co-administration of pools containing two different anti-BoNT/A VHH monomers. (<b>B</b>) Protection from BoNT/A lethality by co-administration of pools containing three different anti-BoNT/A VHH monomers. (<b>C</b>) Protection from BoNT/A lethality by co-administration of pools containing four different anti-BoNT/A VHH monomers. An asterisk indicates that mice did not display any symptoms of intoxication.