Cell-based kinase and Lumicycle assays.

<p>(<b>A</b>) Autoradiography (P³²) and immunoblots of HA-tagged proteins (green) and GFP-tagged CK1δ or CK1ε (red). Graph <i>inset</i> showing percent change of SAPS3 phosphorylation by CK1δ and CK1ε (n = 2). (<b>B</b>) Results from LumiCycle assays in cells transfected with indicated siRNAs. (<b>C</b>) Representative traces after cell synchronization of control (gray), <i>CRY2</i> (green) and <i>PHB2</i> (white) siRNAs (<b><i>left</i></b>). Graph showing effects of control siRNA, <i>CRY2</i> siRNA, two <i>PHB2</i> siRNAs (<i>PHB</i>2.1 & <i>PHB</i>2.2) and three <i>PHB1</i> siRNAs (<i>PHB</i>1.1, <i>PHB</i>1.2, <i>PHB</i>1.3) (<i>right</i>). (Sample size indicated by number inside each bar, ** indicates p<0.001 following regression analysis. Error bars indicate SEM).