Changes in the stem cell-associated markers in LSC after RNA interference.

<p>(A) Representative images of immunofluorescence staining of stem cell associated markers, p63, ABCG2, and integrinβ1, as well as differentiation marker, CK3 in LSC in siRNA-F or siRNA-TERT group. (B) Percentages of positive cells stained for each marker in LSC in siRNA-F or siRNA-TERT group. (C) Quantitative real-time PCR data showing the expression levels (relative fold of mRNA) of these markers by LSC in siRNA-F or siRNA-TERT group. (D)Western blot results showing the protein levels of P63 and ABCG2 in the ESC-CM, CnT-20, siRNA-F or siRNA-TERT groups. Data are expressed as the mean±SD (n = 3;**, p<0.01).