LC3 expression is reduced by HSPB1 small interfering RNA (siRNA) in NRK-LC3 cells.
- Tatsuki Matsumoto (494309)
- Madoka Urushido (737172)
- Haruna Ide (737173)
- Masayuki Ishihara (494310)
- Kazu Hamada-Ode (737174)
- Yoshiko Shimamura (494312)
- Koji Ogata (494313)
- Kosuke Inoue (494314)
- Yoshinori Taniguchi (494315)
- Takafumi Taguchi (737175)
- Taro Horino (494316)
- Shimpei Fujimoto (494319)
- Yoshio Terada (494308)
Publication date
May 2015
DOIAbstract
<p>(A) Western blotting of HSPB1 and LC3 in control or HSPB1 siRNA-transfected NRK-52E cells incubated with 400 μM H<sub>2</sub>O<sub>2</sub> for 4 h. Densitometric analysis of HSPB1 protein and LC3-II protein expression after H<sub>2</sub>O<sub>2</sub> exposure (400 μM). Data are presented as means ± SEM, n = 5; *P < 0.05 vs. control siRNA. (B) Confocal micrograph of green fluorescent protein-positive autophagosomes in HSPB1 siRNA-transfected NRK-LC3 cells incubated with 400 μM H<sub>2</sub>O<sub>2</sub>. All scale bars represent 20 μm.</p
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