Reduction of SPHK expression by RNAi in a panel of cancer cell lines.

<p>Multiple cancer cell lines were transfected in a high-throughput format with libraries of siRNA containing multiple triggers for each gene, and cell viability was determined 96 or 120 hours after transfection. The statistical significance of the observed effects was calculated (see materials and methods) and expressed as a p value. Polo like kinase 1 (PLK1) served as a positive control. Each symbol represents the result of one siRNA screen. Most cell lines were tested several times using different transfection conditions. Symbols to the left of the dashed line (p<0.05) indicate a statistically significant effect of gene knockdown on cell viability in a given experiment.