Mean FRNT₅₀ values for homotypic primary and secondary sera.

<p>Homotypic primary (anti DENV-3) sera (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001486#pntd-0001486-g004" target="_blank">Figures 4A–4H</a>) or secondary serum (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001486#pntd-0001486-g004" target="_blank">Figure 4I</a>) FRNT₅₀ titers against each of the E variant isogenic clones. Each serum sample is identified above the graph. Serum histories are summarized in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001486#pntd-0001486-t002" target="_blank">Table 2</a> and <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001486#pntd.0001486.s001" target="_blank">Text S1</a>. Fold-dilution of serum is on the Y-axis and each clone is identified on the X-axis. Columns show GMT FRNT₅₀ values calculated from FRNT done in triplicate. Error bars indicate 95% confidence intervals. Columns connected by horizontal lines indicate groups of clones that did not have statistically significantly different FRNT₅₀ values (P<0.05). Connecting bars indicate individual or groups of clones that differed statistically (P<0.05) by Tukey's HSD. Sera 003 (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001486#pntd-0001486-g004" target="_blank">Figure 4A</a>), 005 (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001486#pntd-0001486-g004" target="_blank">Figure 4B</a>) and 103 (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001486#pntd-0001486-g004" target="_blank">Figure 4E</a>) did not have significantly different titers against the recombinant viruses.