Longitudinal analysis of DENV-specific serum neutralizing Abs.

<p><b>A.</b> DENV3-specific NT₅₀ of serum day 4–6 (acute), day 14–28 (convalescent), 3 m, 6 m and 18 m post-onset of symptoms in 1° DENV3 infections. Longitudinal serum samples were analyzed via a flow cytometry-based neutralization assay. The mean +/− SD as well as the 25^th and 75^th percentiles of NT₅₀ are displayed. <b>B.</b> DENV3-specific NT₅₀ of serum day 6 (acute), day 14–28 (convalescent), 3 m, 6 m and 18 m post-onset of symptoms in 2° DENV3 infections. Longitudinal serum samples were analyzed as described in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002274#pntd-0002274-g001" target="_blank">Figure 1A</a>. <b>C.</b> DENV2-specific NT₅₀ of serum day 6 (acute), day 14–28 (convalescent), 3 m, 6 m and 18 m post-onset of symptoms in 2° DENV3 infections. Longitudinal serum samples were analyzed as described in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002274#pntd-0002274-g001" target="_blank">Figure 1A</a>. Diamonds, mean; middle line, median; upper and lower boundary of the box, intra-quartile range (IQR; 25th to 75th percentile); whiskers, range of values that are outside of the IQR but are close enough not to be considered outliers (within ≤1.5*IQR); empty circles: outliers >1.5*IQR.