Increased percentage of PB/PCs in peripheral blood of DENV-infected patients.

<p><b>A.</b> Percentage of PB/PCs circulating in the blood of patients suspected of DENV infection. Mean and SE of the percentage of PB/PCs among total lymphocytes plotted according to day post-onset of symptoms. As the same patient may have had up to 3 samples processed, the patient may be represented more than once over time. The number of samples processed is shown below the graph. The percentage of PB/PCs increased over time in DENV-positive samples and peaked at day 5 post-onset of symptoms. Statistical analysis was performed using the Mann-Whitney test, and a significant difference in percentage of PB/PC between OFIs and DENV-positive cases was found at day 5 post-onset of symptoms. The p-value is shown below the symbol legend. <b>B.</b> Percentage of PB/PCs at day 5 post-onset of symptoms in 1° and 2° DENV infections. Statistical analysis was performed using the Mann-Whitney test, and no significant difference in percentage of PB/PCs was found at day 5 post-onset of symptoms between 1° and 2° DENV infections. n = 14 for 1° DENV infections; n = 8 for 2° DENV infections. <b>C.</b> Percentage of memory B cells circulating in the blood of patients suspected of DENV infection. Mean and SE of the percentage of memory B cells plotted according to day post-onset of symptoms. As the same patient may have had up to 3 samples processed, the patient may be represented more than once over time. The number of samples processed is shown below the graph. The evolution over time of the percentage of memory B cells in OFIs and DENV-positive cases is similar. Statistical analysis was performed using the Mann-Whitney test, and a significant difference between OFIs and DENV-positive cases was found on day 4 post-onset of symptoms. The p-value is shown below the symbol legend. <b>D.</b> Percentage of naïve B cells circulating in the blood of patients suspected of DENV infection. Mean and SE of the percentage of naïve B cells plotted according to day post-onset of symptoms. As the same patient may have had up to 3 samples processed, the patient may be represented more than once over time. The number of samples processed is shown below the graph. The evolution over time of the percentage of naïve B cells in OFIs and DENV-positive cases is similar. Statistical analysis was performed using the Mann-Whitney test, and a significant difference between OFIs and DENV-positive cases was found on day 3 post-onset of symptoms. The p-value is shown below the symbol legend. <b>E.</b> Percentage of MZ B cells circulating in the blood of patients suspected of DENV infection. Mean and SE of the percentage of MZ B cells plotted according to day post-onset of symptoms. As the same patient may have had up to 3 samples processed, the patient may be represented more than once over time. The number of samples processed is shown below the graph. The evolution over time of the percentage of MZ B cells in OFIs and DENV-positive cases is similar. Statistical analysis was performed using the Mann-Whitney test, and a significant difference between OFIs and DENV-positive cases was found on day 2 post-onset of symptoms. The p-value is shown below the symbol legend.