Effect of DSS treatment and L-Arg supplementation on L-Arg metabolic pathways.

<p>Mice received 4% DSS ± 1% L-Arg in the drinking water as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033546#pone-0033546-g003" target="_blank">Figures 3</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033546#pone-0033546-g004" target="_blank">4</a>. (A) At sacrifice on day 10, a piece of colon was obtained for RNA isolation or polyamine determination by HPLC. (A–G) Tissue mRNA levels of the genes indicated, as assessed by real-time PCR. (H) Tissue polyamine levels were assessed by HPLC as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033546#s2" target="_blank">Methods</a>. <i>n</i> = 4–15 per group. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001 vs. control. §<i>p</i><0.05 vs. DSS.