Adoptive transfer of female lymphocytes into male mice challenged with TRAMP-C2 controls tumor growth.

<p>A) Adult male mice were myeloablated via irradiation and transplanted with female T-cell depleted bone marrow followed by challenge with TRAMP-C2 tumors 10 weeks after transplantation. n = 5 mice/group, results of a single experiment. B) Adult male mice were sublethally irradiated with 6 Gy and challenged intradermally with 1×10⁶ TRAMP-C2. The following day, mice received a donor lymphocyte infusion (DLI) of 3×10⁷ splenocytes <i>i.v</i>. n = 10 mice/group, one of 3 experiments with similar results. C) Adoptively-transferred CD25+ regulatory T cells do not mediate increased tumor growth seen following male DLI. Mice were irradiated and tumor-challenged as in B, and received splenocytes that were CD25-depleted using magnetic beads where indicated. n = 10 mice/group, results of a single experiment. D) Female CD4 T cells mediate delayed growth of TRAMP-C2 tumors. Mice were irradiated and tumor-challenged as in B, and then received 1.5×10⁷ splenocytes that were <i>in vivo</i> CD4-depleted, mixed with 1.5×10⁷ splenocytes that were CD8-depleted, of either male or female origin. n = 10 mice/group, one of 2 experiments with similar results.